Potential of Endophytic Bacteria from Rambusa Plant as Disease Control and Growth Promoters of Samarinda White Pepper (Piper nigrum L.)

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Published Aug 31, 2024
https://doi.org/10.55043/jaast.v8i3.262
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Vol. 8 No. 3 (2024): Journal of Applied Agricultural Science and Technology

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Nur Hidayat
Samarinda State Agricultural Polytechnic
La Mudi
Samarinda State Agricultural Polytechnic
Taufiq Rinda Alkas
Samarinda State Agricultural Polytechnic
Faradilla Faradilla
Samarinda State Agricultural Polytechnic
F. Silvi Dwi Mentari
Samarinda State Agricultural Polytechni
Daryono Daryono
Samarinda State Agricultural Polytechnic
Ryan Adipratama
Samarinda State Agricultural Polytechnic
Syahrul Wandy
Samarinda State Agricultural Polytechnic

Abstract

Stem base rot (SBR) disease caused by Phytophthora capsici Leonian is associated with significant yield loss. Meanwhile, wild passion fruit (Passiflora foetida L.) interacts with bacteria that have potential to control SBR disease. Therefore, this research aimed to examine potential of endophytic bacteria from wild passion fruit to control SBR disease in pepper plant and the role as growth promoters. A total of 12 endophytic bacteria isolates were tested for potential to promote growth and biocontrol, focusing on the ability to produce the phytohormone IAA, dissolve phosphate, fix nitrogen, as well as produce cellulase and protease enzymes. The three best isolates were then tested on pepper cuttings (Var. Malonan I). The results showed that the highest disease severity was found in control at 40.74% (moderately susceptible), followed by isolate KSA 01 at 37.03%, and SIB 01 at 33.33% (resistant). Disease severity in isolate KPA 03 was significantly lower at 7.41% (very resistant). The highest disease incidence occurred in control and KSA 03 at 44.44%, while the lowest occurred in KPA 03 and SIB 02 at 33.33%. Isolate KPA 03 produced the highest plant height increase (39.87 cm) and the highest number of leaves (5.78), although it was not significantly different from isolate SIB 02. This research showed that endophytic bacteria from wild passion fruit could effectively control SBR disease and improve pepper plant growth.

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Author Biographies

Nur Hidayat, Samarinda State Agricultural Polytechnic

Plantation Crop Cultivation study program

La Mudi, Samarinda State Agricultural Polytechnic

Plantation Crop Cultivation study program

Taufiq Rinda Alkas, Samarinda State Agricultural Polytechnic

Environmental Management study program

Faradilla Faradilla, Samarinda State Agricultural Polytechnic

Plantation Crop Cultivation study program

F. Silvi Dwi Mentari, Samarinda State Agricultural Polytechni

Plantation Crop Cultivation study program

Daryono Daryono, Samarinda State Agricultural Polytechnic

Plantation Crop Cultivation study program

Ryan Adipratama, Samarinda State Agricultural Polytechnic

Plantation Crop Cultivation study program

Syahrul Wandy, Samarinda State Agricultural Polytechnic

Plantation Crop Cultivation study program

How to Cite
1.
Hidayat N, Mudi L, Alkas TR, Faradilla F, Mentari FSD, Daryono D, Adipratama R, Wandy S. Potential of Endophytic Bacteria from Rambusa Plant as Disease Control and Growth Promoters of Samarinda White Pepper (Piper nigrum L.). J. appl. agricultural sci. technol. [Internet]. 2024Aug.31 [cited 2024Dec.8];8(3):413-25. Available from: http://jaast.org/index.php/jaast/article/view/262

References

  1. Paul R. Analysis of area, production, productivity, and export of black pepper with special reference to Kerala. The Pharma Innovation 2023;SP-12:1931–5. https://www.thepharmajournal.com/archives/2023/vol12issue8S/PartY/S-12-8-260-709.pdf
  2. Hidayat N, Andriani F, Mentari FSD, Manullang RR, Awaludin A, Sarie H. Preparation Planting Material by Grafting of Pepper ( Piper nigrum L .) With Malada ( Piper colubrinum L ) for Extensibility in Flooded Land. International Journal of Innovative Science and Research Technology 2021;6:1290–3. https://ijisrt.com/assets/upload/files/IJISRT21MAR288.pdf
  3. Suriany E, Akhsan M. Response of Pepper Seeds Affected by Root Rot Disease (Phytophthora capsici) Towards Application of Secondary Metabolites of Trichoderma sp. Haya: The Saudi Journal of Life Sciences 2021;6:2415–6221. https://doi.org/10.36348/sjls.2021.v06i06.001.
  4. Sofian S, Hadisutrisno B, Priyatmojo A. The Growth of Root Rot Disease on Pepper Seed Applied by Trichoderma Harzianum Inoculum. International Journal of Science and Engineering 2013;5:49–54. https://doi.org/10.12777/ijse.5.1.49-54.
  5. Aravind E., Pervez R., Bhai R.S., Eapen S.J., Kumar A., Sreeja R.K. VEM& KPB. Mitigating Phytophthora foot rot and slow decline diseases of black pepper through the deployment of bacterial antagonists. Journal of Spices and Aromatic Crops 2017:69. https://doi.org/10.25081/josac.2017.v26.i2.874.
  6. Wu P-H, Chang H-X, Shen Y-M. Effects of synthetic and environmentally friendly fungicides on powdery mildew management and the phyllosphere microbiome of cucumber. PLOS ONE 2023;18:e0282809. https://doi.org/10.1371/journal.pone.0282809
  7. Olanrewaju OS, Babalola OO. Bacterial Consortium for Improved Maize (Zea mays L.) Production. Microorganisms 2019;7. https://doi.org/10.3390/microorganisms7110519.
  8. Prihatiningsih N, Djatmiko HA, Lestari P. Antagonistic feature displayed by endophytic bacteria consortium for control rice pathogens. Journal of Tropical Plant Pests and Diseases 2022;22:154–61. https://doi.org/10.23960/jhptt.222154-161.
  9. Glick BR. Plant Growth-Promoting Bacteria: Mechanisms and Applications. Scientifica 2012;2012:963401. https://doi.org/10.6064/2012/963401.
  10. Kesaulya H, Baharuddin, Zakaria B, Syaiful SA. Isolation and Physiological Characterization of PGPR from Potato Plant Rhizosphere in Medium Land of Buru Island. Procedia Food Science 2015;3:190–9. https://doi.org/10.1016/j.profoo.2015.01.021.
  11. Mohanasundari C, Natarajan D, Srinivasan K, Umamaheswari S, Ramachandran A. Antibacterial properties of Passiflora foetida L. – a common exotic medicinal plant. Afr J Biotechnol 2007;6:2650–3. https://api.semanticscholar.org/CorpusID:37206151
  12. Mamangkey EJ, Losung F, Bara RA, Angmalisang PA, Rumampuk NDC, Tumbol R. Isolasi dan Uji Aktivitas Anti Bakteri dari Jamur Simbion dari Teripang (Holothuroidea sp.) yang diambil di Perairan Kelurahan Molas Kecamatan Bunaken Provinsi Sulawesi Utara. JURNAL PESISIR DAN LAUT TROPIS 2022;10:79–88. https://doi.org/10.35800/jplt.10.2.2022.42017.
  13. Triwidodo, H. & Listihani L. Isolation , Selection and Determination of Endophytic Bacteria from Bamboo, Gamal, Tulsi, and Alamanda. Sustainable Environment Agricultural Science (SEAS) 2021;5:151–62. https://www.researchgate.net/publication/356640024_The_ Isolation_Selection_and_Determination_of_Endophytic_Bacteria_from_Bamboo_Gamal_Tulsi_and_Alamanda
  14. Seo WT, Lim WJ, Kim EJ, Yun HD, Lee YH, Cho KM. Endophytic bacterial diversity in the young radish and their antimicrobial activity against pathogens. Journal of Applied Biological Chemistry 2010;53:493–503. https://doi.org/10.3839/jksabc.2010.075.
  15. Kaburuan R, Hapsoh, Gusmawartati. Isolasi Dan Karakterisasi Bakteri Penambat Nitrogen Non-Simbiotik Tanah Gambut Cagar Biosfer Giam Siak Kecil-Bukit Batu. Jurnal Agroteknologi 2014;5:35–9. https://ejournal.uin-suska.ac.id/index.php/agroteknologi/ article/view/1146
  16. Lubis SS, Sardi AS, Huslina FF, Lisa MM. Isolasi dan Karakterisasi Bakteri Pengikat Nitrogen Tanah Gambut Hutan Dari Kecamatan Trumon Aceh Selatan. Quagga: Jurnal Pendidikan Dan Biologi 2020;12:117. https://doi.org/10.25134/quagga.v12i2.2794.
  17. Apine OA, Jadhav JP. Optimization of medium for indole-3-acetic acid production using Pantoea agglomerans strain PVM. Journal of Applied Microbiology 2011;110:1235–44. https://doi.org/10.1111/j.1365-2672.2011.04976.x.
  18. Tahir M, Mirza MS, Hameed S, Dimitrov MR, Smidt H. Cultivation-based and molecular assessment of bacterial diversity in the rhizosheath of wheat under different crop rotations. PLoS ONE 2015;10:1–28. https://doi.org/10.1371/journal.pone.0130030.
  19. Bhattacharyya PN, Jha DK. Plant growth-promoting rhizobacteria (PGPR): emergence in agriculture. World Journal of Microbiology and Biotechnology 2012;28:1327–50. https://doi.org/10.1007/s11274-011-0979-9.
  20. Akhtar MJ, Asghar HN, Shahzad K, Arshad M. Role of plant growth promoting rhizobacteria applied in combination with compost and mineral fertilizers to improve growth and yield of wheat (triticum aestivum l.). Pakistan Journal of Botany 2009;41:381–90. https://www.pakbs.org/pjbot/PDFs/41(1)/PJB41(1)381.pdf
  21. Ghods-Alavi BS, Soleymani M, Ahmadzadeh M, Soleymani S. Ability of rhizobacteria of valerian in phosphate solubilization and their symbiotic efficiency. Journal of Science and Technology Greenhouse Culture 2013;4:2013. https://jspi.iut.ac.ir/browse.php?a_id=447&sid=1&slc_lang=en
  22. Herwati A, Patandjengi B, Jayadi M, Yani A. The Effectiveness of Phosphate Solvent Rhizosphere Bacteria in Stimulating the Growth of Rice Plant. International Journal of Pharmaceutical Research 2021;13:4671–8. https://doi.org/10.31838/ijpr/2021.13.01.613.
  23. Ryan RP, Germaine K, Franks A, Ryan DJ, Dowling DN. Bacterial endophytes: recent developments and applications. FEMS Microbiology Letters 2008;278:1–9. https://doi.org/10.1111/j.1574-6968.2007.00918.x.
  24. Sutariati GAK, Rakian TC, Agustina, Sopacua N, Mudi L, Haq M. Kajian Potensi Rizobakteri Pemacu Pertumbuhan Tanaman Yang Diisolasi Dari Rizosfer Padi Sehat. Jurnal Agroteknos 2014;4:71–7. https://www.neliti.com/publications/243749/kajian-potensi-rizobakteri-pemacu-pertumbuhan-tanaman-yang-diisolasi-dari-rizosf#cite
  25. dos Santos ML, Berlitz DL, Wiest SLF, Schünemann R, Knaak N, Fiuza LM. Benefits Associated with the Interaction of Endophytic Bacteria and Plant. Brazilian Archives of Biology and Technology 2018;61:1–11. https://doi.org/10.1590/1678-4324-2018160431.
  26. PAGARRA H. Isolation and Optimization of Endophytic Bacteria from Roots of Karst Area Ecosystems Producing Protease Enzymes. Journal of Research on the Lepidoptera 2020;51:431–9. https://doi.org/10.36872/LEPI/V51I2/301110.
  27. Rori CA, Kandou FEF, Tangapo AM. Isolasi dan Uji Antibakteri dari Bakteri Endofit Tumbuhan Mangrove Avicennia. Jurnal Bios Logos 2020;1:1–7. https://www.researchgate.net/publication/344164604_Isolasi_dan_Uji_Antibakteri_dari_Bakteri_Endofit_Tumbuhan_Mangrove_Avicennia_marina_Isolation_and_Antibacterial_Test_of_Endophytic_Bacteria_of_Avicennia_marina_Mangroves
  28. Afzal I, Shinwari ZK, Sikandar S, Shahzad S. Plant beneficial endophytic bacteria: Mechanisms, diversity, host range and genetic determinants. Microbiological Research 2019;221:36–49. https://doi.org/10.1016/j.micres.2019.02.001.
  29. Strobel G, Daisy B, Castillo U, Harper J. Natural products from endophytic microorganisms. Journal of Natural Products 2004;67:257–68. https://doi.org/10.1021/np030397v.
  30. Andrade-Linares DR, Franken P. Fungal Endophytes in Plant Roots: Taxonomy, Colonization Patterns, and Functions, 2023, p. 311–34. https://doi.org/10.1007/978-3-642-39317-4_16.
  31. Leonita S, Bintang M, Pasaribu FH. Isolation and Identification of Endophytic Bacteria from Ficus variegata Blume as Antibacterial Compounds Producer. Current Biochemistry 2016;2:116–28. https://doi.org/10.29244/cb.2.3.116-128.
  32. Toghueo RMK. Bioprospecting endophytic fungi from Fusarium genus as sources of bioactive metabolites. Mycology 2020;11:1–21. https://doi.org/10.1080/21501203.2019.1645053.
  33. Wei F, Zhang Y, Shi Y, Feng H, Zhao L, Feng Z, et al. Evaluation of the Biocontrol Potential of Endophytic Fungus Fusarium solani CEF559 against Verticillium dahliae in Cotton Plant. BioMed Research International 2019;2019:3187943. https://doi.org/10.1155/2019/3187943.
  34. Hidayat N, Rajab A, Mudi L. Uji Invitro Daya Hambat Cendawan Endofit Tumbuhan Rambusa (Passiflora foetida) Sebagai Agens Pengendali Hayati Penyakit Layu Fusarium. Jurnal Agrotech 2021;11:64–70. https://doi.org/10.31970/agrotech.v11i2.73.
  35. Kusumawati DE, Pasaribu FH, Bintang M. Aktivitas antibakteri isolat bakt eri endofit dari tanaman mian a ( Coleus scutellariodes [ L .] Benth .) terhadap Staphylococcus aureus dan Escherichia coli. Current Biochemistry 2014;1:45–50. https://journal.ipb.ac.id/index.php/cbj/article/view/17887
  36. Li YH. Phytophthora blight of pepper. Crop Protection 2012:1–2. http://www.informaworld.com/openurl?genre=article&doi=10.1080/09670879209371717&magic=crossref%7C%7CD404A21C5BB053405B1A640AFFD44AE3
  37. Jana SK, Islam MM, Mandal S. Endophytic Microbiota of Rice and Their Collective Impact on Host Fitness. Curr Microbiol 2022;79:37. https://doi.org/10.1007/s00284-021-02737-w.
  38. Mohammed BL, Hussein RA, Toama FN. Biological control of Fusarium wilt in tomato by endophytic rhizobactria. Energy Procedia 2019;157:171–9. https://doi.org/10.1016/j.egypro.2018.11.178.
  39. Ouf SA, El-Amriti FA, Abu-Elghait MA, Desouky SE, Mohamed MSM. Role of Plant Growth Promoting Rhizobacteria in Healthy and Sustainable Agriculture. Egyptian Journal of Botany 2023;63:333–59. https://doi.org/10.21608/ejbo.2023.191783.2246.
  40. Djaya L, Hersanti, Istifadah N, Hartati S, Joni IM. In vitro study of plant growth promoting rhizobacteria (PGPR) and endophytic bacteria antagonistic to Ralstonia solanacearum formulated with graphite and silica nano particles as a biocontrol delivery system (BDS). Biocatal Agric Biotechnol 2019;19:101153. https://doi.org/10.1016/j.bcab.2019.101153.
  41. Hanudin N, Nuryani W, Marwoto B. Induksi Resistensi Tanaman Krisan Terhadap Puccinia Horiana P. Henn. Dengan Menggunakan Ekstrak Tanaman Elisitor. Jurnal Hortikultura 2016;26:245–56. https://doi.org/10.21082/jhort.v26n2.2016.p245-256.
  42. Rashed K. Phytochemical and Biological Effects of Sesamum indicum L.- A Review. Plantae Scientia 2022;5:8–11. https://doi.org/10.32439/ps.v5i1.8-11.
  43. Rosenblueth M, Martínez-Romero E. Bacterial endophytes and their interactions with hosts. Molecular Plant-Microbe Interactions : MPMI 2006;19:827–37. https://doi.org/10.1094/MPMI-19-0827.
  44. Hagaggi NSA, Abdul-Raouf UM. Production of bioactive β-carotene by the endophytic bacterium Citricoccus parietis AUCs with multiple in vitro biological potentials. Microbial Cell Factories 2023;22:1–9. https://doi.org/10.1186/s12934-023-02108-z.
  45. Maoka T. Carotenoids as natural functional pigments. Journal of Natural Medicines 2020;74:1–16. https://doi.org/10.1007/s11418-019-01364-x.
  46. Yadegari M, Rahmani HA, Noormohammadi G, Ayneband A. Plant Growth Promoting Rhizobacteria Increase Growth, Yield and Nitrogen Fixation in Phaseolus vulgaris. Journal of Plant Nutrition 2010;33:1733–43. https://doi.org/10.1080/01904167.2010.503776.
  47. Li S, Yan Q, Wang J, Peng Q. Endophytic Fungal and Bacterial Microbiota Shift in Rice and Barnyardgrass Grown under Co-Culture Condition. Plant (Basel) 2022;11. https://doi.org/10.3390/plant11121592.
  48. Zhao Y, Christensen SK, Fankhauser C, Cashman JR, Cohen JD, Weigel D, et al. A role for flavin monooxygenase-like enzymes in auxin biosynthesis. Science (New York, NY) 2001;291:306–9. https://doi.org/10.1126/science.291.5502.306.
  49. Etesami H, Alikhani HA, Hosseini HM. Indole-3-acetic acid (IAA) production trait, a useful screening to select endophytic and rhizosphere competent bacteria for rice growth promoting agents. MethodsX 2015;2:72–8. https://doi.org/10.1016/j.mex.2015.02.008.
  50. Ma Y, Rajkumar M, Zhang C, Freitas H. Beneficial role of bacterial endophytes in heavy metal phytoremediation. Journal of Environmental Management 2016;174:14–25. https://doi.org/10.1016/j.jenvman.2016.02.047.
  51. Gopalakrishnan S, Srinivas V, Vemula A, Samineni S, Rathore A. Influence of diazotrophic bacteria on nodulation, nitrogen fixation, growth promotion and yield traits in five cultivars of chickpea. Biocatalysis and Agricultural Biotechnology 2018;15:35–42. https://doi.org/10.1016/j.bcab.2018.05.006.
  52. Khamwan S, Boonlue S, riddech N, Jogloy S, Mongkolthanaruk W. Characterization of endophytic bacteria and their response to plant growth promotion in Helianthus tuberosus L. Biocatalysis and Agricultural Biotechnology 2018;13:153–9. https://doi.org/10.1016/j.bcab.2017.12.007.
  53. Valetti L, Iriarte L, Fabra A. Growth promotion of rapeseed (Brassica napus) associated with the inoculation of phosphate solubilizing bacteria. Applied Soil Ecology 2018;132:1–10. https://doi.org/10.1016/j.apsoil.2018.08.017.
  54. Otieno NA, Culhane JK, Germaine KJ, Brazil D, Ryan D, Dowling DN. Screening of large collections of plant associated bacteria for effective plant growth promotion and colonisation. Aspects of Applied Biology 2013:23–8. https://api.semanticscholar.org/CorpusID:85953491
  55. Wiratno, Syakir M, Sucipto I, Pradana AP. Isolation and characterization of endophytic bacteria from roots of piper nigrum and their activities against Fusarium oxysporum and Meloidogyne incognita. Biodiversitas 2019;20:682–7. https://doi.org/10.13057/biodiv/d200310.
  56. Cho KM, Hong SY, Lee SM, Kim YH, Kahng GG, Lim YP, et al. Endophytic bacterial communities in ginseng and their antifungal activity against pathogens. Microb Ecol 2007;54:341–51. https://doi.org/10.1007/s00248-007-9208-3.
  57. Hallmann J, Quadt-Hallmann A, Mahaffee WF, Kloepper JW. Bacterial endophytes in agricultural crops. Can J Microbiol 1997;43:895–914. https://doi.org/10.1139/m97-131.
  58. Sudewi S, Ala A, Baharuddin, Farid M. The isolation, characterization endophytic bacteria from roots of local rice plant kamba in, central sulawesi, indonesia. Biodiversitas 2020;21:1614–24. https://doi.org/10.13057/biodiv/d210442.
  59. Munif A, Wiyono S, Suwarno S. Isolation of Endophytic Bacteria from Upland Rice and Its Role as Biocontrol Agents and Plant Growth Inducer. Jurnal Fitopatologi Indonesia 2012;8:57–64. https://doi.org/10.14692/jfi.8.3.57
  60. Lodewyckx C, Vangronsveld J, Porteous F, Moore ERB, Taghavi S, Mezgeay M, et al. Endophytic Bacteria and Their Potential Applications. CRC Crit Rev Plant Sci 2002;21:583–606. https://doi.org/10.1080/0735-260291044377.
  61. Castanheira NL, Dourado AC, Pais I, Semedo J, Scotti-Campos P, Borges N, et al. Colonization and beneficial effects on annual ryegrass by mixed inoculation with plant growth promoting bacteria. Microbiological Research 2017;198:47–55. https://doi.org/10.1016/j.micres.2017.01.009.
  62. Purwanto UMS, Pasaribu FH, Bintang M. Isolasi Bakteri Endofit dari Tanaman Sirih Hijau (Piper betle L.) dan Potensinya sebagai Penghasil Senyawa Antibakteri. Current Biochemistry 2017;1:51–7. https://doi.org/10.29244/cb.1.1.51-57.
  63. Zinniel DK, Lambrecht P, Harris NB, Feng Z, Kuczmarski D, Higley P, et al. Isolation and characterization of endophytic colonizing bacteria from agronomic crops and prairie plant. Appl Environ Microbiol 2002;68:2198–208. https://doi.org/10.1128/AEM.68.5.2198-2208.2002.