Evaluation of the Physiological Quality of Rice Seeds (Oryza sativa) under Salinity and Pyrite Stress Conditions
##plugins.themes.academic_pro.article.main##
Abstract
Salinity and pyrite are abiotic stress factors that can affect the physiological quality of rice seeds. This study aimed to evaluate the effects of salinity and pyrite stress on the viability and vigor of rice seeds. The research was conducted using a completely randomized design (CRD) with two factors: salinity levels (0 mM, 50 mM, and 100 mM NaCl) and pyrite dosages (0 mg, 200 mg, and 400 mg). The observed parameters included germination, maximum growth potential, vigor index, growth speed, simultaneous growth, and growth rate. The results showed that salinity stress significantly affected the vigor index and growth speed, while pyrite stress only influenced the growth speed. The interaction between the two factors did not show a significant effect on all physiological quality parameters of the seeds. However, there was a tendency for a decline in germination percentage, maximum growth potential, vigor index, growth speed, and simultaneous growth. The combination of high salinity stress (100 mM) and high pyrite dosage (400 mg) caused more pronounced growth retardation, particularly after day 10. Although rice seeds were able to maintain tolerance at low to moderate stress levels, an increase in the intensity of stress from both factors could hinder water and nutrient absorption, thereby reducing overall growth performance. The findings of this study provide insights into the tolerance limits of rice seeds to salinity and pyrite stress, which can serve as a basis for managing suboptimal lands for more sustainable rice production.
##plugins.themes.academic_pro.article.details##

This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
References
- PPID UTAMA. Pemprov Riau Luncurkan Program Opsi Padi Riau Dukung Swasembada Pangan Nasional. Https://PpidRiauGoId/Berita/18424/Pemprov-Riau-Luncurkan-Program-Opsi-Padi-Riau-Dukung-Swasembada-Pangan-Nasional 2024.
- Rustiati T, Susanti Z, Hikmah ZM, Ruskandar A. Pengelolaan Lingkungan Cekaman Salin Untuk Meningkatkan Hasil Padi 2020;8. https://doi.org/10.33603/agroswagati.v6i2.
- Sopandie D. Fisiologi Adaptasi Tanaman terhadap Cekaman Abiotik pada Agroekosistem Tropika. 2013.
- Primayuda A, Suriadikusumah A, Solihin MA. Identifikasi Kedalaman Pirit dan Kaitannya Terhadap Kesehatan dan Produktivitas Tanaman Kelapa Sawit (Elaeis guineensis Jacq.) (Studi Kasus di Perkebunan PT Sawit Sumbermas Sarana Tbk). Jurnal Ilmu Tanah Dan Lingkungan 2022;24:6–13. https://doi.org/10.29244/jitl.24.1.6-13.
- Balai Pengkajian Teknologi Pertanian. Deskripsi Varietas Unggul Baru. 2015.
- Munns R, Tester M. Mechanisms of salinity tolerance. Annu Rev Plant Biol 2008;59:651–81. https://doi.org/10.1146/annurev.arplant.59.032607.092911.
- Chunthaburee S, Dongsansuk A, Sanitchon J, Pattanagul W, Theerakulpisut P. Physiological and biochemical parameters for evaluation and clustering of rice cultivars differing in salt tolerance at seedling stage. Saudi J Biol Sci 2016;23:467–77. https://doi.org/10.1016/j.sjbs.2015.05.013.
- Chanthini KMP, Senthil-Nathan S, Pavithra GS, Malarvizhi P, Murugan P, Deva-Andrews A, et al. Aqueous Seaweed Extract Alleviates Salinity-Induced Toxicities in Rice Plants (Oryza sativa L.) by Modulating Their Physiology and Biochemistry. Agriculture (Switzerland) 2022;12. https://doi.org/10.3390/agriculture12122049.
- Zhang Y, Xu J, Li R, Ge Y, Li Y, Li R. Plants’ Response to Abiotic Stress: Mechanisms and Strategies. Int J Mol Sci 2023;24. https://doi.org/10.3390/ijms241310915.
- Novianti V, Indradewa D, Maryani, Rachmawati D. Enzymatic antioxidant activity and physiological responses of local swamp rice cultivars from Kalimantan-Indonesia under iron toxicity during vegetative stage. J Crop Sci Biotechnol 2023;26:369–86. https://doi.org/10.1007/s12892-022-00187-9.
- Weng J, Xu L, Li P, Xing W, ur Rahman S, Ahmad N, et al. Interactions of Fe and Zn Nanoparticles at Physiochemical, Biochemical, and Molecular Level in Horticultural Crops Under Salt Stress: A Review. Horticulturae 2025;11:442. https://doi.org/10.3390/horticulturae11040442.
- Aculey P, Tandoh PK, Gamenyah DD. Physiological Seed Quality Responses of Three Rice Varieties to Different Storage Materials under Ambient Conditions. Journal of Experimental Agriculture International 2023;45:135–41. https://doi.org/10.9734/jeai/2023/v45i92184.
- Khan MIR, Kumari S, Nazir F, Khanna RR, Gupta R, Chhillar H. Defensive Role of Plant Hormones in Advancing Abiotic Stress-Resistant Rice Plants. Rice Sci 2023;30:15–35. https://doi.org/10.1016/j.rsci.2022.08.002.
- Nurjanah S. Invigorasi Benih Padi Dengan Vermicompost Priming Untuk Meningkatkan Mutu Fisiologis Benih Pada Kondisi Optimum dan Cekaman Salinitas. Skripsi. Institut Pertanian Bogor, 2023.
- Anwar K, Masganti. Pengelolaan Lahan Berpirit di Rawa Pasang Surut Untuk Optimasi Padi. IAARD Press 2021.
- Zhang R, Hussain S, Wang Y, Liu Y, Li Q, Chen Y, et al. Comprehensive evaluation of salt tolerance in rice (Oryza sativa L.) germplasm at the germination stage. Agronomy 2021;11. https://doi.org/10.3390/agronomy11081569.
- Mousuf MTU, Al Mamun MA, Raihan MS, Karim MA. Morpho-Physiological Transformation and Mitigation of Salinity in Rice Cultivation. Ecology Journal 2021;3:217-26. https://www.researchgate.net/publication/372776671_MORPHO-PHYSIOLOGICAL_TRANSFORMATION_AND_MITIGATION_OF_SALINITY_IN_RICE_CULTIVATION
- Gao Y, Zhao X, Liu X, Liu C, Zhang K, Zhang X, et al. OsRAV1 Regulates Seed Vigor and Salt Tolerance During Germination in Rice. Rice 2024;17. https://doi.org/10.1186/s12284-024-00734-8.
- Habibpourmehraban F, Wu Y, Wu JX, Hamzelou S, Masoomi-Aladizgeh F, Kamath KS, et al. Multiple Abiotic Stresses Applied Simultaneously Elicit Distinct Responses in Two Contrasting Rice Cultivars. Int J Mol Sci 2022;23. https://doi.org/10.3390/ijms23031739.
- Marschner P. Mineral Nutrition of Higher Plants. 3rd ed. New York: Academic Press; 2012.
- Singh J, Sharma S, Khanna S, Sharma B, Prasad F. A Study of the Effect of Pyrites and Rhizobium Inoculation on Chlorophyll and Sugar Content in Black Gram under Sodicity Stress Condition. Journal of Environmental Analytical Chemistry 2016;03. https://doi.org/10.4172/2380-2391.1000188. https://www.hilarispublisher.com/open-access/a-study-of-the-effect-of-pyrites-and-rhizobium-inoculation-on-chlorophylland-sugar-content-in-black-gram-under-sodicity-stress-con-2380-2391-1000188.pdf
- Taratima W, Chomarsa T, Maneerattanarungroj P. Salinity Stress Response of Rice (Oryza sativa L. cv. Luem Pua) Calli and Seedlings. Scientifica (Cairo) 2022;2022. https://doi.org/10.1155/2022/5616683.
- Atta K, Pal AK, Jana K. Effects of salinity, drought and heavy metal stress during seed germination stage in ricebean [Vigna umbellata (Thunb.) Ohwi and Ohashi]. Plant Physiology Reports 2021;26:109–15. https://doi.org/10.1007/s40502-020-00542-4.
- Alwi M, Hairani A, Napisah K, Agustina R, Fadhilah N. Yield performance of high-yield rice varieties in swamp lands of the West Kalimantan border area. BIO Web Conf, vol. 155, EDP Sciences; 2025. https://doi.org/10.1051/bioconf/202515501010.
- Aratani H, Rumanti IA, Nugraha Y, Kamiya T, Yamasaki Y, Kato Y. Differences in Fe toxicity response index and associated growth characteristics among rice genotypes. Plant Prod Sci 2023;26:411–7. https://doi.org/10.1080/1343943X.2023.2252146.
- do Nascimento LÁ, Abhilasha A, Singh J, Elias MC, Colussi R. Rice Germination and Its Impact on Technological and Nutritional Properties: A Review. Rice Sci 2022;29:201–15. https://doi.org/10.1016/j.rsci.2022.01.009.
- Wibisono K, Adisyahputra, Azrai EP. Seleksi Toleransi Padi Rawa Terhadap pH Rendah dan Pirit Tinggi Pada Fase Vegetatif Awal. BIOMA 2015;11:88–96. https://journal.unj.ac.id/unj/index.php/bioma/article/view/1325
- Mansoor S, Ali A, Kour N, Bornhorst J, AlHarbi K, Rinklebe J, et al. Heavy Metal Induced Oxidative Stress Mitigation and ROS Scavenging in Plants. Plants 2023;12. https://doi.org/10.3390/plants12163003.
- Krismiratsih F, Winarso S, Slamerto. Cekaman Garam NaCl danTeknik Aplikasi Azolla pada Tanaman Padi. Jurnal Ilmu Pertanian Indonesia (JIPI) 2020;25:349–55. https://doi.org/10.18343/jipi.25.3.349. https://journal.ipb.ac.id/index.php/JIPI/article/view/26880
- Khan MHU, Malook I, Atlas A, Jan M, Jan SU, Shah G. The Effect of Sodium Chloride (NaCl) stress on Seed germination and Seedling Growth of Rice (Oryza Sativa L.). Journal of Bio-Molecular Sciences (JBMS) 2014;2:100-107. https://www.researchgate.net/publication/271525620_The_Effect_of_Sodium_Chloride_NaCl_stress_on_Seed_germination_and_Seedling_Growth_of_Rice_Oryza_Sativa_L
- Mu Y, Li Y, Zhang Y, Guo X, Song S, Huang Z, et al. A comparative study on the role of conventional, chemical and nanopriming for better salt tolerance during seed germination of direct seeding rice. J Integr Agric 2023;23:3998–4017. https://doi.org/10.1016/j.jia.2023.12.013.
- Rahman MA, Rahman MS, Mohiuddin KM, Chowdhury MAH, Chowdhury MAK. Germination and seedling growth of rice (Oryza sativa L.) as affected by varying concentrations of loom-dye effluent. Journal of the Bangladesh Agricultural University 2019;17:153–60. https://doi.org/10.3329/jbau.v17i2.41938.
- Taiz L, Zeiger E. Plant physiology. 3rd ed. Sunderland: Sinauer Associater, Inc., Publishers; 2002. https://fmipa.umri.ac.id/wp-content/uploads/2016/03/Lincoln_Taiz_Eduardo_Zeiger_Plant_PhysiologyBookFi.org_.pdf
- Girija D, Abirami K, Vikrant. Evaluation of Seed Germination and Early Seedling Growth under Heavy Metals Stress Conditions in Coastal Red Rice (Oryza sativa L.) Crop. Journal of Stress Physiology & Biochemistry 2022;18:17–31. http://www.jspb.ru/issues/2022/N3/JSPB_2022_3_17-31.pdf
- Sarma B, Kashtoh H, Tamang TL, Bhattacharyya PN, Mohanta YK, Baek KH. Abiotic Stress in Rice: Visiting the Physiological Response and Its Tolerance Mechanisms. Plants 2023;12. https://doi.org/10.3390/plants12233948.
- Coca LIR, González MTG, Unday ZG, Hernández JJ, Jáuregui MMR, Cancio YF. Effects of Sodium Salinity on Rice (Oryza sativa L.) Cultivation: A Review. Sustainability (Switzerland) 2023;15. https://doi.org/10.3390/su15031804.